Monocyte-Derived Interleukin 1: Effects on Aortic Contraction and Phosphatidylinositol Turnover

Abstract

Medium conditioned by silica-stimulated human peripheral blood monocytes expresses vascular suppressive activity. Rat aortic rings, after incubation in conditioned medium, exhibited compromised contraction to stimulation by norepinephrine (NE). Maximal contraction and sensitivity were both reduced in comparison to contraction and sensitivity displayed by rings after incubation in control medium. A polyvalent antibody (Ab) against human neutralized the suppressive activity in conditioned medium. Rings incubated in conditioned medium containing Ab exhibited normal maximal contraction and a partial restoration of sensitivity to NE. In contrast, incubation of rings in control medium supplemented with recombinant human I1 1 resulted in a dose dependent suppression of aortic contraction to NE that was analogous to the defects induced by monocyte conditioned medium. No significant differences in NE-stimulated hydrolysis of phosphatidylinositol (PI) were present between rings incubated in Ab-treated or untreated contractile function and that the mechanism by which I1 1 induces vascular dysfunction cannot be demonstrated to involve inhibition of NE-PI turnover. Keywords: Sepsis, Human, Rat, Vascular contraction, Phorbol.

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Document Details

Document Type
Technical Report
Publication Date
Nov 29, 1988
Accession Number
ADA210962

Entities

People

  • John E. Lueders
  • Thomas M. Mckenna
  • Wolfgang A. Titius

Organizations

  • Naval Medical Research Center

Tags

DTIC Thesaurus Topics

  • Anti-Bacterial Agents
  • Blood
  • Cells
  • Culture Media
  • Data Analysis
  • Endothelial Cells
  • Immune Serums
  • Lymphocytes
  • Macrophages
  • Monocytes
  • Mrna
  • Muscle Cells
  • Phagocytes
  • Proteins
  • Smooth Muscle
  • Sugar Phosphates
  • Thymocytes

Fields of Study

  • Biology
  • Medicine

Readers

  • Cardiovascular Physiology
  • Immunology