Gel State Chemiluminescence: An Artificial Electron Transport System

Abstract

Crosslinked glucose oxidase and horseradish peroxidase can mimic the macrophage oxidative function in vivo in respect to tumor cells as targets. We used crosslinked glucose oxidase, horseradish peroxidase, and luminol-bovine serum albumin entrapped in filter paper disks to study the chemiluminescent properties of this macrophage-mimicking material. The disks showed a sensitivity to D-glucose down to 1 ug/ml. The luminescence was driven specifically by glucose at pH 6.0, 6.5, 7.0, 7.4, 8.0, and 10.0. The best signal-to-noise ratio, 44,705 to 1, achieved at pH 7.4. Superoxide was produced by the crosslinked system, and the chemiluminescence was dependent on hydrogen peroxide. Keywords: Gel; Peroxidase; Chemiluminescence; Oxidase; Reprints.

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Document Details

Document Type
Technical Report
Publication Date
Jan 01, 1988
Accession Number
ADA211142

Entities

People

  • David M. Simmons
  • David N. Erwin
  • Johnathan L. Kiel

Organizations

  • United States Air Force School of Aerospace Medicine

Tags

Communities of Interest

  • Space

DTIC Thesaurus Topics

  • Aerospace Medicine
  • Air Force
  • Air Force Facilities
  • Albumins
  • Availability
  • Chemiluminescence
  • Classification
  • Corporations
  • Filter Paper
  • Luminescence
  • Materials
  • Protons
  • Radiation
  • Rocket Oxidizers
  • Security
  • Transport Ships
  • Two Dimensional

Readers

  • Chemistry (specifically Chemical Fluorescence)
  • Molecular and Cellular Biochemistry

Technology Areas

  • Microelectronics