Effects of Phorbol Esters and Lipopolysaccharide on Endothelial Cell Microfilaments: Laser Scanning Confocal Microscopy and Quantitative Morphometry of Dose Dependent Changes
Abstract
The disruptive action of phorbol esters on microfilament integrity was used to develop a comparison of dosage effects with effects on the modulation of the phosphoinostitide turnover and protein kinase C regulatory system. The novel technique of laser scanning confocal epifluorescence was used to study fiber orientation in phorbol ester treated cells. Dose-dependence of morphological changes was compared and contrasted to the dose-dependent effect of phorbol esters on bradykinin-stimulated phosphoinositide turnover. PB4DB was less potent in inducing the disruption of microfilament structure than in inhibiting phosphoinostide turnover. Lipopolysaccharide was ineffective in inducing a morphological change under these conditions. A simple activation of protein inase C is insufficient to explain the dose-dependent effects of phorbol esters. Thus a morphometric analysis can help distinguish the potency of modulators. Keywords: Morphometric analysis, Endothelial cells; Laser scanning confocal microscopy, Microfilaments; Phorbol ester fluorescence microscopy; Phosphoinositide turnover.
Document Details
- Document Type
- Technical Report
- Publication Date
- Nov 29, 1988
- Accession Number
- ADA211208
Entities
People
- David K. Wood
- Thor B. Nielsen
Organizations
- Naval Medical Research Center