Oxidative Damage in the Guinea Pig Hippocampal Slice

Abstract

Free radicals and active oxygen compounds are implicated in brain ischemia and head trauma. Previous studies have shown that free radical, generated by radiation and through the Fenton reaction, produced both synaptic and postsynaptic damage in the hippocampal brain slice. To evaluate the contribution of oxidation to the observed damage, the actions of the oxidants, chloramine-T and N-chlorosuccinimide (NCS), were studied on electrophysiological responses in the hippocampal slice isolated from the brains of guinea pigs. Electrical stimulation of afferents to neurons of the CA1 region of hippocampus evoked a population postsynaptic potential (population PSP) in the dendritic layer and a population spike in the cell body layer. These studies suggest that oxidation reactions can account for the synaptic component of the damage produced by free radicals but can not account for the postsynaptic effects. Keywords: Chloramine-T, N-chlorosuccinimide, Oxidation; Free radical; Hippocampus. Reprints.

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Document Details

Document Type
Technical Report
Publication Date
Jan 01, 1989
Accession Number
ADA211335

Entities

People

  • Kathryn L. Neel
  • Terry C. Pellmar

Organizations

  • Armed Forces Radiobiology Research Institute

Tags

DTIC Thesaurus Topics

  • Amplitude
  • Cells
  • Computers
  • Dose Rate
  • Elements
  • Free Radicals
  • Hippocampus
  • Ionizing Radiation
  • Nerve Fibers
  • Nervous System
  • Oxidation
  • Oxygen
  • Oxygen Compounds
  • Peroxides
  • Radiation
  • Rocket Oxidizers
  • Rodents

Fields of Study

  • Biology

Readers

  • Neuroscience
  • Organic Chemistry