Production of Antigens and Antibodies for Diagnosis of Arbovirus Diseases
Abstract
Antigens and sera were produced and standardized for use in the ELISA. Antigens were produced by sucrose-acetone extraction of suckling mouse brain for Oropouche, Bwamba, Tataguine, Dugbe, Hazara, Qalyub, Bandia, Hughes, Charges, Mayaro, Bhanja, Japanese encephalitis, Bunyamwera, Ilheus, Ross River, and Germiston viruses. The antigens were inactivated with beta-propiolactone. Safety tests in suckling mice are not completed. These same viruses were tested in the RK-13 rabbit kidney cell line adapted to grow in rabbit serum. Growth was obtained for Bunyamwera, Japanese encephalitis, Bwamba, Oropouche, Germiston, Bhanja, and Ilheus, but not Qalyub, Ross River, and Charges viruses on serial passage. Rabbits were immunized intravenously with the viruses which grew in RK- 13 cells. Production of rabbit sera and testing of the antigens and antibody in the ELISA are not complete. C6/36 Aedes albopictus cells were adapted to grow in rabbit serum in anticipation of adaptation of the viruses which did not grow in RK-13 cells to this mosquito line. Keywords: Diagnosis medicine; Antigens; Antibodies; Arbovirus; Enzyme linked immunosorbent assay; RA 1.
Document Details
- Document Type
- Technical Report
- Publication Date
- May 08, 1988
- Accession Number
- ADA211363
Entities
People
- Robert E. Shope
Organizations
- Yale University