HTLV 3 Virus Isolation Studies
Abstract
A systematic viral isolation study has been performed on peripheral blood lymphocytes obtained from Air Force personnel positive for antibodies to the human immunodeficiency virus (HIV-1) as assessed by HIV-ELISA and/or immunoblot assays. The co-culture technique employed for viral isolation involves admixing with PHA-activated PBL from normal donors as target cells in the presence of interleukin 2 (IL-2). Assays to determine reverse transcriptase cells (RT) activity in the culture supernatants were used to detect productive viral infectivity in vitro. RT-negative samples were further tested for the presence of HIV-p24 antigen. The results of this viral isolation study in combination with neurological, clinical, and immunological manifestations assessed by the Wilford Hall USAF Medical Center personnel should contribute to our increased understanding of the natural history of the acquired immunodeficiency syndrome. Keywords: Human immunodeficiency virus; Viral isolation; Co-culture; Reverse transcriptase; p24 Antigen capture assay; RA 1.
Document Details
- Document Type
- Technical Report
- Publication Date
- Feb 28, 1989
- Accession Number
- ADA211493
Entities
People
- Tran C. Chanh
Organizations
- Texas Biomedical Research Institute