Evolution of Phosphofructokinase
Abstract
Comparative sequence data suggest that mammalian phosphofructokinase (PFK) has evolved from a procaryotic precursor by gene duplication, fusion, and mutation of previous catalytic sites into new regulatory ligand binding sites. We are using two approaches to examine this problem. We are attempting to duplicate these events by recombinant DNA technology. Using a synthetic oligonucleotide that matches the mammalian link peptide we are joining 2 E. coli PFK genes, analyzing the product, and mutagenizing the product. In the second approach, we are looking at two unique PFKs, a potato enzyme, and one from Propionibacteria to determine their evolutionary path. Despite great overall molecular differences, antibody suggest similarities among the various PFKs.
Document Details
- Document Type
- Technical Report
- Publication Date
- Aug 05, 1989
- Accession Number
- ADA211741
Entities
People
- Robert G. Kemp