Effect of Dengue Virus on Procoagulant and Fibrinolytic Activities of Monocytes
Abstract
Dengue viruses are flaviruses that comprise four distinct types (dengue types 1-4). These viruses are transmitted by the domestic mosquito Aedes aegypti and cause disease with two different clinical manifestations, one mild, i.e., dengue fever(DF); and the other life threatening, leading to shock and known as dengue shock syndrome or dengue hemorrhagic fever (DSS/DHF). DF is typically seen in adults, whereas DHF/DSS occurs predominantly in children. Some of the fibrinolytic and coagulant enzymes that monocytes produce are urokinase, a plasminogen activator (PA); a PA-specific inhibitor (PAI); and procoagulant activity (PCA) which has been characterized as tissue factor. Dengue infection in vivo is restricted to monocytes; however, it is unknown if dengue-infected monocytes undergo alterations in the production of PA, PAI, and PCA activities. This issue was addressed in studies in which monocytes were infected in vitro with dengue 2 virus in serum-free medium in the presence of enhancing antibody. No urokinase activity was detected in either control or infected cells of in their supernatants. Infection of monocytes with the dengue 2 virus resulted in an almost threefold increase in PAI activity in cells and supernatants. No change in relation to the control was observed in PAA generated by the infected cells. These data indicate that dengue 2 infection enhances the production of PAI from monocytes without altering PA or PCA. Reprints.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jun 01, 1989
- Accession Number
- ADA212503
Entities
People
- Barbara Alving
- Chitra Krishnamurti
Organizations
- Walter Reed Army Institute of Research