Structure and Expression of Various RNAs in the Archaebacteria
Abstract
A novel transfer RNA gene of Sulfolobus has been cloned. It has a large 'D loop' containing 27 residues and appears to code for glutamic acid TRNA. The sequence CCGGU occurs twice within the 'D-loop'. Two symmetrical cuts within or just outside these sequences can produce a typical tRNA and an 18-base 'intron'. The gene occurs as a single copy in the genome and is transcribed. It is being determined that whether this 'intron' is actually spliced out or it remains as a part of the mature tRNA. In addition, an approximately 9 Kb EcoRI genomic fragment of Sulfolobus, containing genes for two tRNA-methyltransferases has been cloned. The tRNAs isolated from E. coli cells containing this recombinant plasmid contain N(2)-methylguanosine (m2G) and 1-methyladenosine (m1A), the modified nucleosides not normally present in E. coli tRNAs. It appears that some Sulfolobus genes are expressed when cloned in E. coli and at least some of its enzymes are functional in the E. coli environment. Keywords: Thermophiles, Modified Nucleosides.
Document Details
- Document Type
- Technical Report
- Publication Date
- Sep 05, 1989
- Accession Number
- ADA212667
Entities
People
- Ramesh Gupta
Organizations
- Southern Illinois University Carbondale