Studies of Altered Response to Infection Induced by Severe Injury
Abstract
This research's main goals are to develop assays to monitor the appearance and progression of post-trauma immunosuppression and to examine prophylactic modalities which may reverse or reduce this immunosuppression. This contract year we have successfully adapted assays for IL-1, IL-6, and Transforming Growth Factor Beta(TGFBeta) to use with patient monocytes (M0). We have demonstrated that M0 levels of IL-6 and TGFBeta are elevated in immunosuppression (TGFBeta). Elevation of patients M0's function (IL-6) and maintenance of their immunosuppression (TGFBeta). Elevation of patients M0's production of tumor necrosis factor (TNF) was shown to occur primarily in the cell-associated form of TNF and this elevated TNF occurs concomitant to an elevation in M0 PGE2. Prophylactic treatment with IL-4 was shown to downregulate both TNF and PGE2 while treatment with indomethacin decreased PGE2 but caused increased TNF release. Our data indicated that post-trauma increases in the number of M0 expressing the 72 KD receptor for IgG(FcRI) is correlated to the immunosuppressed patients' M0 increased production of IL-6, TNF, and decreased IL-1 production. Consequently, monitoring the shift in the ratio of patients' FcRI+ to FcRI- M0 may allow detection of post-trauma immune changes without difficult bioassay performance. Keywords: RA II; Monoclonal antibodies; Recombinant DNA; Thermal injury; Infection; Antibody; Leukocytes; Guinea pigs. (kt)
Document Details
- Document Type
- Technical Report
- Publication Date
- Feb 01, 1989
- Accession Number
- ADA213040
Entities
People
- Carol L. Miller-graziano
Organizations
- University of Massachusetts