Characterization by NMR and Fluorescence Spectroscopy of Differences in the Conformation of Non-Aged and Aged Organophosphoryl Conjugates of AChE (acetylcholinesterase)

Abstract

The objective of this project is to detect and characterize, by NMR and fluoressence spectroscopy, differences in the conformation of non-aged and aged organophosphoryl (OP) conjugates of acetylcholinesterase (AChE) and chymotrypsin (Cht). During the period covered by this report, we developed a synthetic procedure to obtain a novel OP which contained the fluorophore, 2- hydroxynaphthol and showed that it could be employed as a covalent inhibitor of both AChE and Cht. We have employed the circular dichroism technique to demonstrate enhancement of the conformational stability of aged OP conjugates of Cht relative to homologous non-aged conjugates. Circular dichroism also revealed conformational differences between aged and non-aged pyrenebutyl-containing OP conjugates of Torpedo AChE in their native state. Keywords: Organophosphate; Acetylcholinesterase; Inhibition; RA 5; Fluorescence; Spectroscopy; 2- Hydroxynaphthol label; Circular dichroism; X-Ray crystallography; Conformation;.

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Document Details

Document Type
Technical Report
Publication Date
Jan 01, 1989
Accession Number
ADA213121

Entities

People

  • I. Silman
  • Y. Ashani

Organizations

  • Israel Institute for Biological Research

Tags

Communities of Interest

  • C4I
  • Weapons Technologies

DTIC Thesaurus Topics

  • Acetylcholinesterases
  • Alcohols
  • Chemical Synthesis
  • Chemistry
  • Crystal Structure
  • Crystallography
  • Crystals
  • Fish
  • Organic Chemistry
  • Organophosphates
  • Security
  • Spectra
  • Three Dimensional
  • X Rays
  • X-Ray Crystallography

Fields of Study

  • Chemistry

Readers

  • Molecular and Cellular Biochemistry
  • Neurotoxicology