Different Patterns of Inositol Polyphosphate Production Are Seen in B lymphocytes after Cross-Linking of sIg by Anti-Ig Antibody or by a Multivalent Anti-Ig Antibody Dextran Conjugate
Abstract
The current experiments were undertaken to determine whether the differences observed in the mitogenicity mediated by two agonists were also reflected in different patterns, kinetics and magnitudes of inositol polyphosphate production. Although both dextran conjugated and unconjugated anti-Ig antibodies induced rapid increases in level of I(1,4,5)P3, dextran conjugated anti-IG induced much greater levels of this isomer, which were sustained for significantly longer periods of time. Additional differences were found in the amount and in the ratio of the other inositol polyphosphates that were stimulated. This suggests that the distribution of PIP hydrolysis products that are stimulated by cross-linking if sIg is strongly dependent on the extent and type of cross-linking of sIg that is induced. Keywords: Reprints; Bells; Lymphocytes; Antigens; Immunoglobulin. (KT)
Document Details
- Document Type
- Technical Report
- Publication Date
- Sep 01, 1989
- Accession Number
- ADA213215
Entities
People
- Carl H. June
- Fred D. Finkelman
- James J. Mond
- Mark Brunswick
Organizations
- Naval Medical Research Center