Use of Monoclonal Antibodies to Study the Structural Basis of the Function of Nicotinic Acetylcholine Receptors on Electric Organ and Muscle and to Determine the Structure of Nicotinic Acetylcholine Receptors on Neurons
Abstract
During the past year we continued studies of nicotinic acetylcholine receptors of both muscle and neuronal types. Muscle-type acetylcholine receptors were studied in a human cell line, in Torpedo electric organ, using cDNAs for subunits of electric organ receptor expressed in yeast, and by chemical synthesis of parts of Torpedo receptor subunits. Neuronal nicotinic receptors extracted from brain membranes were characterized biochemically, fragments of receptor subunits were expressed in bacteria, and in collaborative experiments neuronal nicotinic receptors were localized histologically using monoclonal antibodies. Neuronal alpha-bungarotoxin-binding protein has been used to begin a library of monoclonal antibodies, and two candidates for subunit cDNAs have been sequenced and are being tested. Keywords: Bioassay; Nicotinic acetylcholine receptors of muscle; Synthetic peptides; Nicotinic acetylcholine receptors of neurons; Protein sequencing; Protein purification; RAI; RAV; Monoclonal antibodies. (kt)
Document Details
- Document Type
- Technical Report
- Publication Date
- Mar 16, 1989
- Accession Number
- ADA213241
Entities
People
- Jon M. Lindstrom
Organizations
- Salk Institute for Biological Studies