Primary Events in Olfactory Receptiom
Abstract
The following major discoveries are reported on herein: 1 We have established procedures for the dissociation and reassembly of ciliary membranes from olfactory epithelium under conditions that allow survival of the olfactory adenylate cyclase. These studies represent a first step toward the molecular dissection and functional reconstitution of the chemosensory membrane. 2 We have discovered that the olfactory adenylate cyclase is regulated by calcium/ calmodulin and characterized the effects of calcium/calmodulin on modulation of this enzyme. 3 We have characterized proteins that are substrates for cyclic AMP-dependent protein kinase in ciliary and axonal membranes of olfactory receptor cells. In addition, we have identified a protein of which the phosphorylation proceeds independent of cyclic nucleotides and is inhibited by low concentrations of calcium. 4 We have established a library of monoclonal antibodies against olfactory cilia and identified several epitopes that are unique to chemosensory cilia and absent from olfactory nerve membranes, respiratory cilia and membranes from a host of other tissues. Keywords: Olfaction, Smell, Cyclic AMP, Signal transduction, Monoclonal antibodies.
Document Details
- Document Type
- Technical Report
- Publication Date
- Oct 10, 1989
- Accession Number
- ADA213649
Entities
People
- Robert R. Anholt
Organizations
- Duke University Hospital