Further Development and Validation of the Frog Embryo Teratogenesis Assay - Xenopus (Fetax)

Abstract

The goal of the project was to develop and validate the Frog Embryo Teratogenesis Assay Xenopus (FETAX). FETAX is a 96 h whole embryo bioassay designed to rapidly assess the developmental toxicity of pure compounds or complex mixtures. The four specific objectives of this study are: 1) validate FETAX using direct-acting compounds of known mammalian developmental toxicity, 2) develop and validate an in vitro metabolic activation system using rate liver microsomes, 3) explore the use of three carrier solvents which will help solubilize non-polar compounds and, 4) develop an atlas of malformations which will assist users in performing FETAX. Five test compounds were selected for FETAX validation during the first year. Each compound was to be tested using 1-2 range finding tests and three definitive tests. Endpoints of FETAX are mortality, malformation and growth inhibition. Frog embryo teratogenesis assay-xenopus, Developmental toxicology, Teratology, Validation metabolic activation system. (JES)

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Document Details

Document Type
Technical Report
Publication Date
May 12, 1989
Accession Number
ADA213868

Entities

People

  • John A. Bantle

Organizations

  • Oklahoma State University–Stillwater

Tags

Communities of Interest

  • Biomedical

DTIC Thesaurus Topics

  • Alcohols
  • Anti-Bacterial Agents
  • Biological Sciences
  • Biomedical Research
  • Cells
  • Chemical Synthesis
  • Chemistry
  • Complex Mixtures
  • Culture Techniques
  • Deoxyribonucleic Acids
  • Enzyme Inhibitors
  • Microsomes
  • Organic Chemistry
  • Pharmacology
  • Plastic Explosives
  • Range Finding
  • Toxicity

Readers

  • Computational Modeling and Simulation
  • Toxicology/Environmental Toxicology