Human Red Blood Cell Freezing With and Without Metabolizable Cryopreservatives, Molecular Distillation Drying, Storage, and Subsequent Rehydration. Phase 1.

Abstract

Studies to determine the optimum choice of freezing and drying methods for fresh human erythrocytes have demonstrated that cryoprotection and anhydrous stabilization with 10-12% (w/v) combinations of sucrose and raffinose prior to slow-rate cooling at -5 C/min, followed by molecular distillation drying at rates over a relatively broad range, with subsequent rehydration in liquid medium at 20-37 C, result in the best structural preservation and minimum cell fragmentation or clumping. (KT)

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Document Details

Document Type
Technical Report
Publication Date
Oct 26, 1989
Accession Number
ADA213971

Entities

People

  • S. Randolph

Tags

Communities of Interest

  • Materials and Manufacturing Processes

DTIC Thesaurus Topics

  • Blood Cells
  • Cell Count
  • Cells
  • Climate Change
  • Contracts
  • Cooling
  • Corporations
  • Cryoprotective Agents
  • Distillation
  • Electron Microscopy
  • Erythrocytes
  • Freezing
  • Glycerols
  • Microscopy
  • Phase Transformations
  • Propylene Glycol
  • Transmission Electron Microscopy

Readers

  • Analytical Chemistry
  • Immunology