Mutagenic Responses of some Petroleum-Base Obscurants in the Ames Test
Abstract
In the Ames test, five petroleum fractions diesel fuel (DF) 25% distilled, DF with approximately 6% tetraethoxysilane, Jet-A, 75% bottoms product and Fog Oil, and methylene chloride extractions of combusted DF were evaluated for their mutagenic potential. The five fractions were dissolved in ethanol containing 1% Tween 80. One hundred milligrams of the residue from combusted DF was extracted with 25 mL of methylene chloride for 24 hr. After that time, the methylene chloride was replaced with acetone by evaporation. Each of five materials was tested in the four standard tester strains; TA97, TA98, TA100, and TA102, metabolically activated (aroclor 1254 induced rat liver S9) and nonactivated. Five concentrations, ranging upwards to the approximate level of cytotoxicity, of each material were tested, and the results in each case were confirmed with a similar nonconcurrent test. Positive results were not demonstrated in any of the materials tested. Keywords: Ames test; Metabolic activation; Mutagenicity; Cytotoxicity; Cancer; Tetraethoxysilane; Fog oil; Jet-A fuel; 75% bottoms product; Combusted diesel fuel; Diesel fuel 25% distilled; Extracts.
Document Details
- Document Type
- Technical Report
- Publication Date
- Sep 01, 1989
- Accession Number
- ADA214201
Entities
People
- Bernard J. Brown
- Fred K. Lee Jr.
- William T. Muse Jr.
Organizations
- Edgewood Chemical Biological Center