Control of Cytokine Gene Expression in Macrophages: Role of Second Messenger Pathways
Abstract
The overall objective of this project is to enhance our understanding of the intracellular second messenger pathways involved in the regulation of the expression of soluble cellular mediator (cytokine) genes in macrophages by bacterial lipopolysaccharide (LPS) and interluekin-2 (IL-2). One aim of this study is to directly examine the mechanisms of action of LPS and IL-2 on the expression of two potent immunomodulatory and cytotoxic cytokines (interleukin-1 (IL-1) alpha and beta and tumor necrosis factor alpha (TNFalpha)). During Year 1, an analysis of the second messenger pathways involved in the expression of IL-1beta mRNA has been initiated. These studies involved a comparison of protein kinase C and calmodulin kinase involved in the transduction of signals initiated by IL-2 and LPS which lead to the expression of IL-1beta mRNA in human monocytes. Another aim of the project involves an examination of the expression of fibrogenic cytokines, including transforming growth factor-beta (TGFbeta), platelet-derived growth factor (PDGF) and fibroblast growth factor (FGF), which have been implicated in models of wound healing and fibrosis. Studies initiated in the past several months have examined the kinetics of expression of TGFbeta, PDGF A chain and PDGF B chain mRNAs in monocypes.
Document Details
- Document Type
- Technical Report
- Publication Date
- Nov 01, 1989
- Accession Number
- ADA214590
Entities
People
- Elizabeth J. Kovacs
Organizations
- Stritch School of Medicine