Development of an Assay for the Early Detection of Organ Specific Carcinogenesis by the Determination and Turnover of NAD(+) and Polyadenosine Diphosphoribose
Abstract
A hypothesis was proposed that predicts the possibility of developing a specific biochemical methodology capable of detecting early and specific signals in chromatin proteins that precede cellular toxicity or malignant transformation, caused by toxic agents or by other environmental factors (e.g., radiation). This long-range aim has been approached the identification of covalent modification of chromatin proteins by a specific enzymatic process of ADP-ribosylations and poly (ADP)-ribosylation to be the most probable biochemical area that can signal toxic effects leading to either cellular damage or malignant transformation. The experimental basis of this hypotheses is: a.) the immunochemical and more recently chemical localization and determination of chromosomal non-histone proteins as specific nuclear acceptors of ADP-ribose and of its polymers; b.) by the detection of specific perturbations in ADP- ribosylations of non-histone proteins during early carcinogenesis, development and hormonal influences, biological areas that are recognized to express differentiated cellular processes. Since the signals obtained during early carcinogenesis, development and hormone actions are clearly distinguishable, there is a predictably high probability that highly specific macromolecular mechanisms will become identifiable during subsequent research. Keywords: Actin, Biosynthesis, Hormones, Aldosterone, Enzyme inhibitors, Hypophysectomy, Adrenolectomy.
Document Details
- Document Type
- Technical Report
- Publication Date
- Oct 28, 1980
- Accession Number
- ADA214793
Entities
People
- Ernest Kun
Organizations
- University of California, San Francisco