Effect of Bacterial Endotoxin and Interleukin-2 on Human Leu-11(+) NK cells: Ultrastructural and Functional Correlations
Abstract
Bacterial endotoxin (lipopolysaccharide, LPS) and interleukin 2 (IK- 2) are known to stimulate NK cell mediated cytotoxicity against tumor cells. In the present report we sought to correlate the stimulatory effect of IL-2nd LPS on NK cells with ultrastructural changes which occurred as a result of such stimulation. Peripheral blood mononuclear cells (PBMC) were purified from healthy donors by a Ficoll-Hypaque density gradient technique. Leu-11+ NK cells were isolated by flow microfluorometry using a monoclonal FITC conjugated anti- Leu-11a antibody and a FACS II cell sorter. The PBMC were incubated respectively with E. coli LPS or recombinant IL-2 (rIL-2) for various time periods. Sorted Leu-11+ NK cells were incubated with LPS for 24 hrs. The NK cytotoxicity contained within the PBMC and sorted LEU-11+ cells was assessed by a 51 Cr release technique using K562 tumor cells are targets. Reprints.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jan 01, 1988
- Accession Number
- ADA214839
Entities
People
- D. M. Carl
- Lorrita P. Watson
- Yuan-hsu Kang
Organizations
- Naval Medical Research Center