Inhibition of ACTH Release by Peptide Hormones: Molecular Mechanisms and Possible Role as Anti-Stress Factors
Abstract
The major objective of this proposal was to investigate whether somatostatin (SRIF) may be a non-steriod inhibitor of ACTH release. The goals of the proposal were to examine the structure of the SRIF receptor and determine whether glucocorticoids regulate the expression of the SRIF receptor in pituitary. In our previous reporting period, we showed that glucocorticoid downregulate the pituitary SRIF receptor. This downregulation could involve a sequestration of the receptor or an inhibition of the synthesis of SRIF receptor. To be able to examine the synthesis and expression of the SRIF receptor, we are presently trying to clone the gene encoding the SRIF receptor. This is being attempting using two approaches. First we have purified the receptor to homogeneity and are attempting to sequence it. Second, we were recently able to express the receptor in oocytes and are using this capability for expression cloning of the receptor. Furthermore, we have been able to demonstrate the functionally distinct subtypes of SRIF are expressed. One receptor preferentially binds SRIF-14 while the other binds to SRIF-28. Attempts are underway to determine the molecular basis for these SRIF receptor subtypes. (kt)
Document Details
- Document Type
- Technical Report
- Publication Date
- Nov 16, 1989
- Accession Number
- ADA215212
Entities
People
- Terry Reisine
Organizations
- University of Pennsylvania