Effects of Immunomodulatory Drugs on T Lymphocyte Activation and Function
Abstract
During this third reporting year (5/15/88 to 5/14/89), the following drugs were tested for their immunomodulatory actions. Acridine trihydrochloride (CL246), OK432, WY18251, poly ICLC (AVS1761), Quinolinamine (AVS1300), and Ampligen (AVS2149). These drugs were tested for their effects on the production of antibody that is stimulated upon addition of Pokeweed Mitogen (PWM) to lymphocyte cultures or upon infection of lymphocytes with Epstein-Barr Virus (EBV). The drugs were also tested for their effects on lymphocyte proliferation stimulated by EBV. Finally, the drugs were tested for their ability to modulate the generation of lymphokine activated killer cells (LAK) which are stimulated upon culturing T lymphocytes with Interleukin 2 (IL2). We found that Acridine trihydrochloride had a pronounced inhibitory effect on all of the above tested functions. This inhibition was most pronounced with drug concentrations above 0. 1 micro g/ml. Quinolinamine at concentrations above 0.1 micro g/ml stimulated both the PWM-stimulated lgM and lgG production, as well as the EBV-induced proliferation of lymphocytes. This drug had also a small, but significant augmenting effect on EBV-induced lgM production at > 1 micro g/ml without, however, affecting the virus-induced lgG production. Finally, at low concentrations (0.001-1 micro g/ml) Quinolinamine stimulated the induction of LAK cells while at higher concentrations (2.5-20 micro g/ml) inhibited their production
Document Details
- Document Type
- Technical Report
- Publication Date
- Jun 15, 1989
- Accession Number
- ADA216047
Entities
People
- Constantine Tsoukas