The Design of Oligonucleotides Which Attack Specific Gene Targets

Abstract

During the first year of Navy support, we have refined our understanding of triple helix formation and in the process, have synthesized a set of triplex forming oligonucleotides directed against sites in the control region of interesting eukaryotic genes. We have shown that, in favorable instances, the dissociation constant for triplex formation can exceed 10-9M under physiological conditions. Work has begun to extend those structural analyses, by application of molecular modeling and 2D NMR methods. Work has also begun to apply photochemical analysis of triplex formation for the purpose of mapping triplex formation on gene target sites (HPRT and EGFR) within intact eukaryotic cells. Keywords: DNA recognition; Triplex helix formation.

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Document Details

Document Type
Technical Report
Publication Date
Dec 08, 1989
Accession Number
ADA216496

Entities

People

  • Michael E. Hogan

Organizations

  • Baylor College of Medicine

Tags

DTIC Thesaurus Topics

  • Biochemistry
  • Cell Line
  • Cells
  • Chemistry
  • Classification
  • Contracts
  • Dissociation
  • Gene Expression
  • Growth Factors
  • Identification
  • Recognition
  • Security
  • Skin Diseases
  • Spectroscopy
  • Standards
  • Structural Analysis
  • Universities

Fields of Study

  • Chemistry

Readers

  • Molecular Genetics
  • Organic Chemistry
  • Systems Analysis and Design