Role of Protein Phosphorylation in the Regulation of Neuronal Sensitivity

Abstract

This report covers studies three principal topics: (A) Establishing differentiated NG108-15 cells (NG cells) grown in culture as a model system for studying the role of protein phosphorylation in the regulation of neuronal function and neuronal adaption. (B) Characterizing ecto-protein kinase activity and its endogenous substrates in neural cells, and determining their role in regulating receptor sensitivity. (C) Raising monoclonal antibodies against specific neuronal phosphoproteins, with emphasis on the 54KDa substrate of a GTP-preferring protein kinase, and the substrates of ectokinase activity. These antibodies will be used in experiments designed to provide direct evidence for the function of these phosphoproteins. (aw)

Open PDF

Document Details

Document Type
Technical Report
Publication Date
Mar 29, 1985
Accession Number
ADA216510

Entities

People

  • Yigal H. Ehrlich

Organizations

  • University of Vermont

Tags

Communities of Interest

  • Biomedical

DTIC Thesaurus Topics

  • Abstracts
  • Air Force
  • Antibodies
  • Biochemistry
  • Cell Membrane
  • Cells
  • Cellular Structures
  • Chemical Synthesis
  • Chemistry
  • Classification
  • Column Chromatography
  • Cultured Cells
  • Gel Electrophoresis
  • Immune Serums
  • Nervous System
  • Proteins
  • Synapses

Fields of Study

  • Biology

Readers

  • Cellular and Molecular Pathways of Apoptosis.
  • Molecular and Cellular Biochemistry
  • Neuroscience