Fluorescence and Elastic Scattering from Laser Dye-Filled Capillaries
Abstract
Capillary Zone Electrophoresis (CZE) is a newly developing field of analysis whereby laser excitation causes biological materials in a hollow core capillary to fluoresce. The fluorescence is then studied to identify constituent elements and/or characterize specific properties in the core materials under investigation. We investigated the elastic scattering and fluorescence from laser dye solutions inside 5000, 1100, and 96.5 micron inner-diameter hollow-core capillaries. Incident 4416 laser illumination of Coumarin 7 dye dissolved in ethanol caused fluorescence from approximately 4600 to 6000. This was studied over an angular range from 0 deg to 360 deg. A light scattering nephelometer coupled with a spectrometer gave intensity measurements as functions of wavelength (at fixed detection angles) and angle (at fixed wavelengths), while the illumination source, dye-filled capillary, and detector remained stationary. We saw capillary size and detection-angle dependence of the fluorescence and elastic scattering. Results show that angular variations of the elastic scattering and emitted fluorescence can be used to determine an optimum detection angle from the capillary with respect to the incident illumination direction. Keywords: Laser induced fluorescence; Helium-cadmium laser; Electrophoresis; Spectroscopy. Theses.
Document Details
- Document Type
- Technical Report
- Publication Date
- Aug 23, 1989
- Accession Number
- ADA217026
Entities
People
- Edward M. Sekerak
Organizations
- University of Arizona