Quantitation of Alkaline Phosphatase Isoenzymes Using Agarose Containing Wheat Germ Lectin
Abstract
Alkaline phosphatase (AP), orthophosphoric monoester phosphohydrolase is present in almost all tissues of the human body and has long been recognized by physicians as an indicator of bone and/or liver disease. This report lists a wide range of tissues which have AP in their cells. Alkaline phosphatase in plasma originated primarily from liver, bone, intestine and placental tissues. These different tissues enzymes are all membrane bound and in the presence of magnesium ion will hydrolyze phosphate monoesters at an alkaline pH (1). This common property gives them the name isoenzyme because it refers to an enzyme from different origins or forms with the same enzymatic activity. The term 'isoenzyme', as defined by the International Union of the Biochemistry Commission on Biochemical Nomenclature (2), refers to different forms of an enzyme catalyzing the same substrate and all having genetically different primary structures. Structural differences due to post translational modification are referred to as isoforms. Theses.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jul 01, 1989
- Accession Number
- ADA217251
Entities
People
- Russell J. Meiling
Organizations
- Air Force Institute of Technology