DURIP - Upgrade of the Meridian ACAS-470 for Toxicological Research

Abstract

This proposal was to have our current Meridian ACAS 570 laser cytometer upgraded with a 5 watt coherent laser, Olympus microscope with a CCD camera and video monitor, a mass data storage computer and dual detector system. This upgrade allowed us new dimensions of research potentials for studying mechanisms of chemical-induced toxicities in living cells, specifically enabling us to detect if chemicals caused toxicities by altering free Ca++ level, causing pH changes or induction of free radicals in single cells. During this year, we have pioneered the application of this new instrument in the demonstration that (a) heptachlor, a toxic pesticide/neurotoxicant, caused increased of free Ca++; (b) several cytotoxic chemicals could generate free radicals; and (c) oncogene products could be detected using fluorescent antibodies; and (d) UV-induced DNA damage could be quantified using fluorescent antibodies. Keywords: Laboratory equipment; Meridian ACAS-570; Laser image analyzer; Quantitative fluorescence detection; Mechanisms of chemical toxicities.

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Document Details

Document Type
Technical Report
Publication Date
Jan 18, 1990
Accession Number
ADA217683

Entities

People

  • James E. Trosko

Organizations

  • Michigan State University

Tags

Communities of Interest

  • Biomedical

DTIC Thesaurus Topics

  • Cell Line
  • Cell Membrane
  • Cells
  • Chemical Synthesis
  • Chemistry
  • Detectors
  • Epithelial Cells
  • Fatty Acids
  • Intercellular Junctions
  • Laser Beams
  • Organic Chemistry
  • Peptides
  • Proteins

Readers

  • Optical Physics and Photonics.
  • Research Science/Academic Research
  • Toxicology/Environmental Toxicology

Technology Areas

  • Directed Energy