Development of Shuttle Vectors for Halobacteria

Abstract

We continue to develop tools for genetic analysis in the archaebacterium Halofera (Halobacterium volcanii). This includes (1) development of transformation systems, (2) construction and tailoring of shuttle vectors, (3) genetic construction of host strain to reduce residual problems associated with restriction and recombination, and (4) application to a variety of genetic analyses. Among such applications are detailed characterization of the halobacterial HMGCoA reductase gene and of clusters of genes involved in the biosynthesis of tryptophan, histidine, arginine and leucine. Our techniques have allowed construction of a genetic map with several dozen markers, and will permit detailed study of archaebacterial gene structure and function. (JG)

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Document Details

Document Type
Technical Report
Publication Date
Feb 01, 1990
Accession Number
ADA217764

Entities

People

  • W. F. Doolittle

Organizations

  • Dalhousie University

Tags

DTIC Thesaurus Topics

  • Amino Acids
  • Anabolism
  • Archaea
  • Bacteria
  • Biology
  • Cells
  • Chemistry
  • Coding
  • Genetic Techniques
  • Genetics
  • Microbial Genetics
  • Microbiology
  • Microorganisms
  • Molecular Biology
  • Notation
  • Nova Scotia
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Fields of Study

  • Biology

Readers

  • Molecular Genetics

Technology Areas

  • Biotechnology