Regulation of Brain Neuropeptide Secretion by Lymphokines

Abstract

Infectious stress is associated with a variety of phenomena, such as changes in pituitary hormone secretion, that may involve the regulatory peptides somatostatin (SRIF) and CRF. This project was undertaken to examine the effects of cytokines upon the synthesis and release of these peptides in rat brain cell cultures. Our studies revealed that recombinant cytokines, including interleukin-1 and tumor necrosis factor, are able to stimulate the synthesis and release of SRIF from primary cultures of fetal diencephalon and cortex. This stimulation is not detectable over periods of minutes or hours, but develops more slowly. Increases in SRIF and CRF mRNA are detectable at 24 hours, with increases in SRIF peptide detectable shortly thereafter. IL-1 and TNF are synergistic in their effects and stimulation is not observed with interleukin-2. Cytokine activity was accompanied by marked stimulation of cell proliferation, as quantitated by tritiated thymidine incorporation, and temporarily suppressed by inhibition of proliferation with cytosine arabinoside.

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Document Details

Document Type
Technical Report
Publication Date
Jan 12, 1990
Accession Number
ADA217766

Entities

People

  • David E. Scarborough

Tags

Communities of Interest

  • Biomedical

DTIC Thesaurus Topics

  • Biological Factors
  • Brain
  • Cells
  • Central Nervous System
  • Culture Techniques
  • Growth Factors
  • Health Services
  • Hormones
  • Nervous System
  • Neurodegeneration
  • Neuroglia
  • Peptide Growth Factors
  • Peptides
  • Pituitary And Hypothalamic Hormones And Analogues
  • Proteins

Fields of Study

  • Medicine

Readers

  • Cellular and Molecular Pathways of Apoptosis.
  • Immunology
  • Neuroscience