An Endotoxin Binding Protein for Treatment of Septic Shock
Abstract
In the first quarter of this project, our primary goal was to purchase the equipment and supplies necessary to assemble the scaled-up extraction process for the endotoxin binding protein. Two large, 150 liter, stainless steel tanks were fitted with sterile connections to serve as pyrogen free buffer reservoirs. Now, buffers are prepared in advance to fill the 150 liter polypropylene extraction tank with its motor driven propeller. To date, one half of the frozen cell debris has been extracted in 6 Molar Urea and clarified by diatomaceous earth filtration. A housing and membrane cassettes for ultrafiltration have also been purchased and used to filter the clarified extract. We have processed all of the extract to date through the 30K dalton filter and concentrated it with a 8K dalton filter.
Document Details
- Document Type
- Technical Report
- Publication Date
- Dec 31, 1989
- Accession Number
- ADA217918