Observations on a Venom Neutralizing Albumin Enriched Fraction Isolated from Serum of the Northern Copperhead Agkistrodon Contortrix Mokasen

Abstract

Venom neutralizing capacity of serum from the northern copperhead Agkistrodon contortrix mokasen was studied. Crude serum neutralized the hemorrhagic, proteolytic and lethal activity of A. c. mokasen venom. The serum neutralized up to 2.5 Lethal dose, 50% of A. c. mokasen venom and up to 5 LD50 of A. piscivorus venom. The serum was antihemorrhagic and a potent inhibitor of venom proteases and trypsin. An albumin enriched fraction was isolated from A. c. mokasen serum by DEAE-Affi-Gel blue chromatography and Superrose-12 (molecular sieve) fast protein liquid chromatography with recycling. This fraction contained two major proteins with apparent molecular weights of 62 kD and 79 kD respectively and several contaminants with an Mr from 52-79 kD. The fraction had a pl of 4.6-4.7 and displayed anodal migration in horizontal electrophoresis. (jg)

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Document Details

Document Type
Technical Report
Publication Date
Jan 31, 1990
Accession Number
ADA218033

Entities

People

  • Leonard A. Smith
  • Pierre J. Lafaye
  • Scott A. Weinstein

Organizations

  • United States Army Medical Research Institute of Infectious Diseases

Tags

Communities of Interest

  • Biomedical

DTIC Thesaurus Topics

  • Albumins
  • Animals
  • Biomedical Research
  • Blood
  • Blood Proteins
  • Cardiovascular Physiological Phenomena
  • Cells
  • Chemistry
  • Enzyme Inhibitors
  • Flow Rate
  • Hemorrhage
  • Lethal Dosage
  • Molecular Sieves
  • Molecular Weight
  • Observation
  • Resistance
  • Rodents

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