Structure and Characterization of the Ion-Channel of the Nicotinic Acetylcholine Receptor
Abstract
The nicotinic acetylcholine receptor (AcChR) is a complex of four polypeptides of molecular weight 40,000, 50,000, 60,000 and 65,000 daltons, generally referred to as alpha, Beta, gamma, sigma, which we first described (1) and this has since been confirmed in several laboratories (2). By performing amino-terminal amino acid sequence analysis of these four polypeptides we demonstrated that they are a highly homologous set of proteins (3) presumably derived from a single ancestral gene and further that they are associated with a stoichiometry of alpha, Beta, gamma, sigma in AcChR from the electric ray, Torpedo californica, the most convenient source of this receptor molecule. Based on the amino acid sequence data we obtained, four groups constructed nucleotide probes for the isolation of C-DNA for subsequent sequence determination. (kt)
Document Details
- Document Type
- Technical Report
- Publication Date
- Jul 01, 1984
- Accession Number
- ADA218380
Entities
People
- Michael A. Raftery
Organizations
- California Institute of Technology