Antibody-Dependent Enhancement of Dengue Virus Growth in Human Monocytes as a Risk Factor for Dengue Hemorrhagic Fever
Abstract
Serum specimens collected during a prospective study of dengue infections among schoolchildren in Bangkok were tested for their ability to enhance dengue 2(DEN-2) virus growth in human monocytes in vitro. Two groups of dengue-immune sera were compared: 32 dengue antibody positive serum specimens from children who subsequently developed asymptomatic secondary dengue infections; and 9 dengue antibody positive serum specimens from children who subsequently developed severe symptomatic secondary dengue infections, 8 of which were clinically diagnosed as dengue hemorrhagic fever. Dengue antibodies can be neutralizing and therefore protective, or they can be enhancing and increase the risk of dengue hemorrhagic fever. In the current study, we sought to determine if differences in serum ADE activity in preinfection sera from seropositive children could be related to the severity of subsequent infection. We report here that, among dengue-immune children, high serum DEN-2 ADE activity was a strong predictor of severe illness. Reprints.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jan 01, 1989
- Accession Number
- ADA218616
Entities
People
- Amanda Nisalak
- Donald S. Burke
- Larry Wahl
- Srisakul C. Kliks
- Walter E. Brandt
Organizations
- Walter Reed Army Institute of Research