Isolation of a New Herpesvirus from Human CD4+ T Cells

Abstract

A new human herpesvirus has been isolated from CD4(+) T cells purified from the peripheral blood mononuclear cells of a healthy individual (RK), following incubation of the cells under conditions promoting T-cell activation. The virus could not be recovered from nonactivated cells. Cultures of lymphocytes infected with the RK virus exhibited a cytopathic effect, and electron microscopic analyses revealed a characteristic herpesvirus structure. RK virus deoxyribonucleic acid did not hybridize with large probes derived from herpes simplex virus, Epstein-Barr virus, varicella-zoster virus, and human cytomegalovirus. The genetic relatedness of the RK virus to the recently identified T-lymphotropic human herpesvirus 6 (HHV-6) was investigated by restriction enzyme analyses using 11 probes derived from two strains of HHV-6 (Z29 and U1102). Keywords: Human herpesvirus, CD4+ Human T cells, Latency, T cell activation, Virus latency, Reprints, Microbiology.

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Document Details

Document Type
Technical Report
Publication Date
Jan 01, 1990
Accession Number
ADA219088

Entities

People

  • Ehud Roffman
  • Eric C. Schirmer
  • George Katsafanas
  • Linda S. Wyatt
  • Niza Frenkel

Organizations

  • Naval Medical Research Center

Tags

DTIC Thesaurus Topics

  • Blood
  • Deoxyribonucleic Acids
  • Dna Virus Infections
  • Dna Viruses
  • Electron Microscopes
  • Electron Microscopy
  • Electrons
  • Health Services
  • Herpesviridae
  • Herpesviridae Infections
  • Infection
  • Lymphocytes
  • Microscopes
  • Viral Structures
  • Virus Diseases
  • Viruses
  • Wound Infections

Fields of Study

  • Biology

Readers

  • Immunology
  • Molecular Genetics
  • Virology (or Medical Virology).

Technology Areas

  • Biotechnology
  • Biotechnology - Cancer Biotech
  • Microelectronics