Channel Protein Engineering: A Novel Approach towards the Molecular Dissection Determinants in Ligand-Regulated Channels
Abstract
Our goal is to establish the molecular determinants that account for the pore properties of ligand-regulated channel properties. We are modeling hypothesized pore forming structures from acetylcholine receptors using semi- empirical potential energy functions. The M2(delta) segment of the nicotinic acetylcholine receptor from Torpedo californica is a candidate for the channel lining structure. It forms ionic channels in human erythocyte membranes and in lipid bilayers. Furthermore, we designed and synthesized a tethered tetramer containing four M2(delta)-oligopeptides. The complete 101 residue protein forms channels in lipid bilayers with properties that resemble those of authentic cholinergic receptors. The general validity of this approach is currently being assessed. (JG)
Document Details
- Document Type
- Technical Report
- Publication Date
- Feb 15, 1990
- Accession Number
- ADA219134
Entities
People
- Mauricio Montal
Organizations
- University of California, San Diego