Effects of Collection Methods and Storage on the In Vitro Stability of Canine Plasma Catecholamines
Abstract
The purpose of the study reported here was to use HPLC to measure NE and EPI degradation in plasma stored at -70 C and at 24 C from heparinized or EDTA plasma in the presence or absence of reduced glutathione (GSH) and EGTA. In addition, NE and EPI degradation in blood at 24 C was determined. Norepinephrine (NE) and epinephrine (EPI) collected from dogs were sequentially and temporally measured in blood and plasma at 24 C. Heparin and EDTA anticoagulants, in combination with reduced glutathione and EDTA as a preservative, were also compared. Norepinephrine and EPI concentrations were measured by high-pressure liquid chromatography with electrochemical detection. In heparinized plasma, NE and EPI concentrations were relatively stable in the absence or presence of preservative after 24 hours at 24 C. In EDTA plasma, NE and EPI values were less stable when compared with those in heparinized samples. Keywords: Veterinary medicine; Reprints.
Document Details
- Document Type
- Technical Report
- Publication Date
- Feb 01, 1990
- Accession Number
- ADA219362
Entities
People
- Dale F. Gruber
- H. L. Reed
- Kevin P. O'halloran
- Michele M. D'alesandro
- Robert Robertson
Organizations
- Naval Medical Research Center