A Sensitive and Specific DNA Probe for Plasmodium yoelii

Abstract

We have produced a DNA probe, designated pJC104, for the sensitive and specific identification of Plasmodium yoelii. The probe incorporates the major DNA repeat of the parasite and has a sensitivity that exceeds that of all conventional methods of malaria diagnosis. The probe represents a major breakthrough for the Navy's malaria vaccine development program, as it provides a means for quantifying the protective effects of experimental immune interventions in the mouse -- P. yoelii system. Specifically, protection against challenge infection by passive transfer of antibody, adoptive transfer of immune cells, infusion of bio-active substances and immunization with subunit vaccines can now be reliably measured. Plasmodium yoelii, Malaria diagnosis, DNA probe, Vaccine screening.

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Document Details

Document Type
Technical Report
Publication Date
Dec 01, 1989
Accession Number
ADA219411

Entities

People

  • J. R. Campbell

Organizations

  • Naval Medical Research Center

Tags

DTIC Thesaurus Topics

  • Biomedical Research
  • Blood
  • Cells
  • Classification
  • Culture Techniques
  • Detection
  • Filter Paper
  • Immune System
  • Infection
  • Lymphocytes
  • Materials
  • Paper
  • Proteins
  • Scintillation Counters
  • Sensitivity
  • Vaccines
  • Wound Infections

Fields of Study

  • Biology
  • Medicine

Readers

  • Immunology
  • Oncology and Biomarker-Based Cancer Detection.
  • Parasitology and Pharmacology of Malaria.

Technology Areas

  • Biotechnology