Chemical Inhibition of Rabbit Sperm Cell Motility in Toxicological Testing
Abstract
The use of motile rabbit spermatozoa to assess the potential cytotoxic effect of chemicals was investigated. A suspension of motile sperm cells in a defined medium was incubated at 37 C under an atmosphere of 5% Carbon Dioxide 8% diatomic oxygen and 87% diatomic nitrogen with three different concentrations of the test chemicals. At specified time intervals, the motion characteristics of the cells were observed by phase microscopy (100x). The test chemicals were ranked in order of their ability to inhibit rabbit cell motility. There was a good correlation between this ranking, the performance of the chemicals in the Draize test, and the inhibition by the chemicals of the in vitro incorporation of amino acids and uridine by mouse 3T3 cells. The inhibition of rabbit sperm cell motility shows promise as a method for in vitro assessment of cytotoxicity. (AW)
Document Details
- Document Type
- Technical Report
- Publication Date
- Jan 01, 1990
- Accession Number
- ADA219488
Entities
People
- David Burnett
- Ronald J. Young
Organizations
- Edgewood Chemical Biological Center