LP1845 Liquid Gun Propellant Dermal Toxicity Studies: CHO/HGPRT Gene Mutation Assay of LP1846 Liquid Gun Propellant
Abstract
The purpose of this study was to evaluate LP1846 Liquid Gun Propellant for toxicity and mutagenic activity at the hypoxanthine-guanine phosphoribosyl transferase (HGPRT) locus in Chinese hamster ovary (CHO) cells. Mutant (HGPRT-) cells were identified by their resistance to 6-thioguanine. LP1846 was evaluated in experiments in the presence and absence of an exogenous metabolic activation system (S9). The cells were treated with culture medium containing the test sample (240 to 1000 micrograms per millimeter) for 4 hr. In these experiments, the average cloning efficiencies relative to solvent controls of cultures treated with 1000 micrograms per millimeter LP1846 were 61% without S9 and 53% with S9. The criteria for a positive response was a threefold or greater increase in the frequency of 6-thioguanine-resistant colonies, compared with the solvent control cultures, in treated cultures with an average initial survival rate of at least 20%. LP1846 did not induce a concentration-dependent increase in mutant frequency either without or with S9 and was evaluated as negative under both activation conditions. RA 3; LP1846 Liquid gun propellant; CHO/HGPRT gene; Mutation assay; Dermal toxicity; Cells(Biology); Ammunition and explosives; Chemical exposure.
Document Details
- Document Type
- Technical Report
- Publication Date
- Mar 12, 1990
- Accession Number
- ADA219649
Entities
People
- Colette J. Rudd
- Pamela S. Lee
Organizations
- SRI International