Characterization of an Opioid-Like Hibernation Induction Trigger
Abstract
A new autoperfusion multiorgan preparation has been developed in our laboratories in which the dogs are anesthetized and artificially ventilated. The heart, lungs, liver, pancreas, duodenum and both kidneys are removed en bloc while being perfused by the heart and oxygenated by the lungs. A respirator is used for ventilation. Fresh blood, electrolytes, antibiotics and other nutrients are infused by intravenous dripping. No anticoagulants or inotropic drugs are necessary. The heart continues to pump blood to all the organs, and the lungs oxygenate the blood. The liver keeps its normal biological functions. Electrolyte and water balances are maintained, and metabolic wastes are removed by the kidneys. This preparation preserves the natural anatomic, as well as physiologic, connections of the organs without foreign material and requires little intervention. In the original study, liver congestion reduced survival time to an average of 14.8 hours. However, when plasma from deeply hibernating woodchucks, containing a hibernation induction trigger (HIT), was infused into the multiorgan autoperfusion system, an average survival time of 44 hours was obtained under non-sterile conditions. Studies of HIT and DADLE, utilizing the multiorgan autoperfusion system preservation, may establish a role for these agents in treatment of multiorgan system failure, clinical organ preservation, trauma management, and related applications. Moreover, the extension of organ survival, utilizing the multiorgan system, may serve as a rapid assay for HIT- activity of resolved plasma fractions. Keywords: RA 2; Hibernation Induction Trigger (HIT); Woodchucks; Delta opioid; DADLE; Multiorgan autoperfusion system. (kt)
Document Details
- Document Type
- Technical Report
- Publication Date
- Jul 01, 1989
- Accession Number
- ADA219743
Entities
People
- Peter R. Oeltgen
Organizations
- University of Kentucky