Determination of the Structural Basis of Antibody Diversity Using NMR
Abstract
A group of twelve hybridomas have been prepared that secrete monoclonal antibodies against a paramagnetic nitroxide spin label hapten. The CDNA sequences of both chains of all twelve IgG antibodies have been determined. NMR difference spectra together with specific amino acid deuteration permit the observation of almost high resolution NMR spectra of amino acid residue protons in the combining site regions. Composition, structural and kinetic information concerning these combining sites has been obtained. Single single site mutations are in progress to confirm signal assignment and for structure-function studies. There appears to be an excellent correspondence between the NMR results and x- ray crystallographic data on the Fab fragment of antibody AN02. Keywords: DNA; Codon DNA; Molecular structure; Binding sites.
Document Details
- Document Type
- Technical Report
- Publication Date
- Mar 15, 1990
- Accession Number
- ADA219803
Entities
People
- Harden M. Mcconnell
Organizations
- Stanford University