Monovalent Ligands of Complement Receptor 2 Inhibit Whereas Polyvalent Ligands Enhance Anti-Ig-Induced Human B Cell Intracytoplasmic Free Calcium Concentration

Abstract

We have performed experiments to investigate the role of ligands for complement receptor 2 (CR2) in human B cell activation. Flow microfluorimetry was used to assess changes in free intracytoplasmic calcium concentration Ca(2+) in indo-loaded B cells, immediately after exposure to anti-micron antibody and to monovalent or polyvalent CR2 ligands. As monovalent ligands we used the C3d fragment and synthetic C3 peptides (peptides P14, residues 1201-1214, and P28, residues 1187-1214). As polyvalent ligands we used i) an intact monoclonal mouse anti-CR2 antibody (HB5) and its F(ab'2) fragment, ii) tetravalent P13 ((residues 1202-1214) 4-template), and iii) P28 conjugated to BSA (molar ratio 5/1). B cell, Complement receptor 2, Antigen receptor, Cell calcium.

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Document Details

Document Type
Technical Report
Publication Date
Mar 01, 1990
Accession Number
ADA220029

Entities

People

  • Carl H. June
  • Evangelos D. Anastassiou
  • Fred D. Finkelman
  • George C. Tsokos
  • John D. Lambris

Organizations

  • Naval Medical Research Center

Tags

Communities of Interest

  • Biomedical

DTIC Thesaurus Topics

  • Abstracts
  • Allergy And Immunology
  • Antibodies
  • B Lymphocytes
  • Blood
  • Blood Cells
  • Cell Membrane
  • Cell Physiological Processes
  • Cells
  • Identification
  • Immune System
  • Lymphatic System
  • Lymphocytes
  • Molecules
  • Polysaccharides
  • Proteins
  • Tissues

Fields of Study

  • Biology

Readers

  • Molecular and Cellular Biochemistry
  • Organic Chemistry

Technology Areas

  • Fully Networked C3