Granulocyte-Macrophage Colony-Stimulating Factor: More Than a Hemopoietin
Abstract
Granulocyte-macrophage colony-stimulating factor (GM-CSF) is a glycoprotein with hormone-like properties and naturally occurring molecular mass between 14,500 and 35,000. The base polypeptide has 127 amino acids and its heterogeneity of molecular mass has been attributed to the degree of glycosylation. The glycosylation varies with the cell source but there does not appear to be specific biological roles for the different molecular species. Three different forms of the molecule have been cloned, produced in large quantities, and are currently under investigation in clinical trials. These recombinant forms are derived from a mammalian cell line (COS cells), bacterial cells (Escherichia coli), and yeast, each with varying degrees of glycosylation. The in vitro biological properties of these molecules does not appear to be different. This review is organized to present the effects of GM-CSF on normal hematopoiesis and its effect on mature myeloid cells. This will be followed by the results of clinical trials and the future applications of GM-CSF therapy. Keywords: Immunology, Cytokines, hemopoietins, Reprints.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jan 01, 1990
- Accession Number
- ADA220076
Entities
People
- Rodney L. Monroy
- Thomas A. Davis
- Thomas J. Macvittie
Organizations
- Naval Medical Research Center