Quarterly Report for Contract N00014-88-C-0755 (California University)
Abstract
The purpose of this study was to find out if blood that had been frozen and thawed could be stored in a refrigerator for periods longer than 72 hours without excessive hemolysis, and if transfused, would have an acceptable in-vivo survival. Currently we have drawn 75 donors and have studied 36 of these with in-vitro analysis. In this quarter, 11 units have been studied in which the entire unit was stored in the same anticoagulant to determine if the changes found in the smaller bags occurred in whole units of blood. To determine this, a full unit of blood was drawn from a single donor, frozen, thawed. ACD and CPDA-1 showed a fairly acceptable hemolysis level over a period of 28 days as typified by the slow rise in plasma hemoglobin and potassium. On the other hand the additive solutions AS-1 (the equivalent of Fenwal's Adsol Solution) and AS-3 (the equivalent of Cutter's Nutracell Solution) showed considerable hemolysis. When studying the CPD anticoagulant 2 of the 3 units of blood showed results very comparable to those found both with ACD and CPDA-1. However, the third unit exhibited an accelerated hemolysis and for this hemolysis was not typical of the storage medium. It would therefore appear that any of these three anticoagulants is acceptable for storing a unit for longer periods of time than 72 hours if sterility is assured.
Document Details
- Document Type
- Technical Report
- Publication Date
- Mar 28, 1990
- Accession Number
- ADA220210
Entities
Organizations
- University of California, Los Angeles