Molecular Basis of Paralytic Neurotoxin Action on Voltage-Sensitive Sodium Channels

Abstract

In years 1 - 5 of this project, progress was made on several objectives: A. The sites and mechanisms of action on the sodium channel were examined and further defined for three new classes of neurotoxins: Goniopora toxins, Brevetoxins, and Conotoxins. B. Monoclonal antibodies with high affinity for the mammalian neuronal sodium channel were developed and methods to screen them for activity at neurotoxin binding sites were established. C. Site-directed antibodies against defined regions of the amino acid sequence of the sodium channel were prepared and shown to bind at discrete negatively charged subsites on the extracellular surface of the channel that may form part of neurotoxin receptor sites. Keywords: Ion transport; Sodium channels; Action potentials; Electrical excitability; Neurotoxins; RA 1.

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Document Details

Document Type
Technical Report
Publication Date
Oct 20, 1989
Accession Number
ADA221018

Entities

People

  • William A. Catterall

Organizations

  • University of Washington

Tags

DTIC Thesaurus Topics

  • Amino Acids
  • Biochemistry
  • Biomedical Research
  • Cell Line
  • Cells
  • Chemistry
  • Coding
  • Culture Techniques
  • Identification
  • Membrane Potentials
  • Molecular Weight
  • Mrna
  • Neurons
  • Physiological Effects
  • Recognition
  • Scorpions
  • Sialic Acids

Fields of Study

  • Biology

Readers

  • Molecular and Cellular Biochemistry