Effect of Microcystin-LR on Cultured Rat Endothelial Cells
Abstract
Primary cultures of adult rat hepatic sinusoidal endothelial cells were used to investigate the effect of microcystin-LR. Microcystin-LR at a concentration (4 microns), which induces necrosis in cultured rat hepatocytes, did not produce either permeability changes, or cytotoxicity in endothelial cell monolayers. However, supernatants derived from cultured rat hepatocytes treated with microcystin-LR induced significant permeability changes, as indicated by the release of adenine nucleotides, and a small reduction of cell density in endothelial cell monolayers. Thus the effect of microcystin-LR on liver sinusoidal endothelial cells in vitro was an indirect one; hepatocytes treated with microcystin-LR produced either an activated metabolite(s) or other factors that affected endothelial cells. Indirect endothelial cell injury may contribute to microcystin-LR-induced liver hemorrhage observed in vivo. (JES)
Document Details
- Document Type
- Technical Report
- Publication Date
- Feb 26, 1990
- Accession Number
- ADA221168
Entities
People
- G. W. Anderson Jr.
- J. Hewetson
- K. Mereish
- R. Solow
Organizations
- United States Army Medical Research Institute of Infectious Diseases