Purification of the Alpha Glycerophosphate Oxidase from African Trypanosomes

Abstract

The a-glycerophosphate oxidase (GPO) system has been investigated in order to purify this important terminal oxidase from African trypanosomes. Using Trypanosoma brucei, a purification scheme was developed that employed a 6-amino hexanoic acid Sepharose 4B gel to which trypan blue was attached. A- glycerophosphate releases this binding allowing elution of the GPO. The 6- aminohexanoic acid 'spacer arm' on the Sepharose 4B facilitates removal of the GPO from the gel column. An estimation of the purification of GPO achieved using this scheme is approximately 1500X. Key problems in this purification scheme include sufficient amounts of starting material and the identification of more efficient affinity substrates. Keywords: Glycerolphosphate; Trypanosomes; Sleeping sickness; A-glycerophosphate oxidase; Ubiquinol oxidase; Affinity chromatography; Sepharose.

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Document Details

Document Type
Technical Report
Publication Date
Feb 02, 1987
Accession Number
ADA221525

Entities

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  • George C. Hill

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  • Meharry Medical College

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  • Biomedical

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  • Biology

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