Chinese Journal of Biotechnology

Abstract

Fragments larger than 4 kb from Sau3A1 partial digested plasmid DNA of Bacillus thuringiensis subsp. kenyae 7404 as well as subsp. kurstaki HD-1 were cloned respectively into the BamH I site of pBR322. Based on the results of in situ colony hybridization, radioimmune screening and Western blot analysis, four transformants containing the corresponding endotoxin gene and producing proteins reacted with crystal protein antibody were selected. Upon biological toxicity tests, out of three transformants tested, the lysate of one transformant TK89 carrying endotoxin gene of B.T. 7407 and two transformants TH12 and TH48 carrying endotoxin gene of HD-1 were toxic to caterpillars of tobacco budworm, (Heliothis assulta). This is the first time to have cloned the endotoxin gene of B.T. subsp kenyae different in serotype with the well studied subsp. Keywords: Bacillus thuringiensis; DNA cloning, Endotoxin, China.

Open PDF

Document Details

Document Type
Technical Report
Publication Date
Apr 02, 1990
Accession Number
ADA221573

Tags

Communities of Interest

  • Biomedical

DTIC Thesaurus Topics

  • Anti-Bacterial Agents
  • Antibodies
  • Bacteria
  • Biological Toxins
  • Biotechnology
  • Blood
  • Diarrhea
  • Digestive System Processes
  • Endotoxins
  • Engineering
  • Gel Electrophoresis
  • Genetic Engineering
  • Immune Serums
  • Materials
  • Military Medicine
  • Proteins
  • Toxicity

Fields of Study

  • Biology

Readers

  • Microbial Pathology
  • Molecular Genetics

Technology Areas

  • Biotechnology