Ultrastructural Correlates of Sulfur Mustard Toxicity

Abstract

Standardized ultrastructural technology was used to study subcellular effects of sulfur mustard (HD) on (1) human lymphocytes in vitro, (2) human keratinocytes in culture, and (3) the skin of the hairless guinea pig. While doses of mustard differed according to the dictates of individual protocols, all specimens were gathered 24 hours following exposure, aldehyde fixed, and processed for transmission and scanning electron microscopy. In the hairless guinea pig model the subcellular effects on basal cells of the stratum germinativum and the generation of microblisters at the dermal-epidermal junction were unequivocal to that reported for human-skin grafted to cogenitally athymic nude mice. Keywords: Sulfur mustard (HD), Toxicity, Lymphocytes in vitro, Keratinocytes in culture, Skin, Ultrastructure, Pathology.

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Document Details

Document Type
Technical Report
Publication Date
Oct 01, 1989
Accession Number
ADA221617

Entities

People

  • John P. Petrali
  • Kenneth R. Mills
  • Susan B. Oglesby

Organizations

  • United States Army Medical Research Institute of Chemical Defense

Tags

DTIC Thesaurus Topics

  • Biological Sciences
  • Biomedical Research
  • Blood
  • Boundaries
  • Cell Membrane
  • Cell Membrane Structures
  • Cells
  • Cellular Structures
  • Classification
  • Electron Microscopy
  • Lymphocytes
  • Microscopy
  • Military Research
  • Organelles
  • Rodents
  • Scanning Electron Microscopy
  • Transmission Electron Microscopy

Fields of Study

  • Biology
  • Medicine

Readers

  • Analytical Chemistry
  • Immunology
  • Trauma Surgery or Emergency Medicine.

Technology Areas

  • Microelectronics