Purification of the Alpha Glycerophosphate Oxidase from African Trypanosomes

Abstract

The bloodstream forms of African trypanosomes are completely dependent on glycolysis for their energy supply and utilize a unique shuttle, glycerophosphate oxidase, which includes a terminal oxidase, to reoxidize the glycolytically produced NADH. This terminal oxidase which is located in the mitochondrial membrane is cytochrome independent and not inhibited by classical inhibitors of the respiratory chain. This enzyme complex consists of two components: a flavin-linked glycerol-3-phosphate dehydrogenase and a glycerol-3- phosphate oxidase (GPO) which are probably linked by ubiquinol. The oxidase component of the enzyme is absent in the mammalian host and is specifically inhibited by salicylhydroxamic acid. We describe here the partial purification of GPO from the bloodstream from of African trypanosomes. Keywords: Glycerolphosphate; Trypanosomes; Sleeping sickness; A-glycerophosphate oxidase; Ubiquinol oxidase; Affinity chromatography; Sepharose.

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Document Details

Document Type
Technical Report
Publication Date
Oct 28, 1989
Accession Number
ADA221637

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  • George C. Hill

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  • Meharry Medical College

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